Analysis Note

Negative ControlNormal mouse serum

Positive ControlSkin cells or tissue

Application

Immunoblotting (1 g/ml, chemiluminescence)Immunoprecipitation (2 g/ml lysate)Paraffin Sections (4-8 g/ml, heat pre-treatment required)

General description

Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.

Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.

This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.

Immunogen

semi-purified, human vinculin

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.

Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol.79, 961.Bakolitsa, C., et al. 1999 Cell99, 603.Rudiger, M., et al. 1998 Bioessays20, 733Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131Otto, J.J. 1990 Cell Motil. Cytoskeleton16, 1.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Warning

Toxicity: Standard Handling (A)